Simultaneous determination of 16 mycotoxins in cereals ...

2017-5-24 · Simultaneous determination of 16 mycotoxins in cereals using stable isotope labeled internal standards and e—Method by UHPLC-MS/MS Introduction Mycotoxins are toxic secondary metabolites of fungi, which can occur in various cereals, including corn, wheat, and barley. Due to the complexity of food

Determination of Regulated and Emerging Mycotoxins in ...

Mycotoxins can occur naturally in a variety of food products via pre- and post-harvest contamination mechanisms and are regulated worldwide. Herein we describe a multi-toxin method for 31 regulated and emerging mycotoxins using a quick and simple pass-through SPE clean-up prior to LC-MS/MS analysis for cereal-based products, ground- and tree-nuts, dried figs, and …

Multiple Mycotoxin Determination on Tunisian Cereals …

2020-3-28 · Twenty-two mycotoxins were investigated in bread and biscuits samples (n = 115) purchased from Tunisia. Deoxynivalenol (DON), enniatin B (ENB), ENB1, and zearalenone (ZEA) occurred in the samples with ENB detected in all the samples. Whole bread was the most contaminated matrix showing the highest amounts of DON (25.2 ng/g), ENB (10.7 ng/g), and …

Simultaneous Determination of Multi-Mycotoxins in Cereal ...

An improved analytical method compared with conventional ones was developed for simultaneous determination of 13 mycotoxins (deoxynivalenol, nivalenol, 3-acetylnivalenol, aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, fumonisin B1, fumonisin B2, T-2, HT-2, zearalenone, and ochratoxin A) in cereal grains by liquid chromatography-tandem mass spectrometry …

Analytical methods for determination of mycotoxins: An ...

Mycotoxins are a problematic and toxic group of small organic molecules that are produced as secondary metabolites by several fungal species that colonise crops. They lead to contamination at both the field and postharvest stages of food production with a considerable range of foodstuffs affected, from coffee and cereals, to dried fruit and spices.

Analysis methods for mycotoxins

(An individual analysis of these mycotoxins is also possible using the method codes: AFL, DON, FUM, T2, OTA or ZEA) [MYC] Multi-method for the determination of mycotoxins in animal feed, cereals and cereal products, coffee, dried fruits and nuts – supplementary package. [MYC_02_A]

Challenges of mycotoxins and their control

2021-5-26 · After developing the determination can be achieved with long-wave UV-light (Holcomb et al., 1992). Various applications of TLC are described (Turner et al., 2009). Both types - one-dimensional and two-dimensional TLC - are used for quantitative and semi-quantitative determination of mycotoxins (Lin et al., 1998).

Mycotoxin Analysis IV Section 7

2020-7-22 · Mycotoxins are natural poisons produced by fungi as secondary metabolites. Foods and animal feeds may become contaminated with mycotoxins as a result of mold growth during harvest or storage.

Rapid and sensitive detection of mycotoxins by advanced ...

For accurate and rapid determination of these mycotoxins in unprocessed cereals and cereal-based products, sensitive, analytical methods are highly relevant to the toxicological implications to animals and humans and highly desirable in order to measure risk of exposure, further to confirm regulatory levels fixed by the United States, European ...

Multiplexed mycotoxins determination employing white …

2020-4-1 · Taking into account that according to EU legislation, the maximum levels (MLs) in μg/kg or ppb for AFB 1 for maize destined for human consumption are 5.0 ppb, while for FB 1 in unprocessed maize and maize based cereals and snacks are 800 and 4000 ppb, respectively, the proposed sensor is suitable for the determination of the two mycotoxins in ...

Assessment of Mycotoxin Exposure in a Rural County of ...

2.3. Occurrence and Concentration of Mycotoxins in Urine. The prevalence and mean (standard deviation (SD)) concentration of mycotoxins in urine, along with the limits of detection (LOD) and quantification (LOQ) of the method, are shown in Table 3.The analysis of urine samples showed that DON was the most frequently occurring mycotoxin (73%), with 63% of the samples above …

Detection and determination of natural toxins (mycotoxins ...

2012-1-1 · A method for the determination of AFs in peanuts and OTA in cereals using MSPD as clean-up and the analysis of Fusarium toxins in cereals using the QuEChERS method have been published (Blesa et al., 2003, Blesa et al., 2004, Zachariasova et al., 2010).

Mycotoxin detection

2016-2-1 · Emerging and masked mycotoxins are identified and detected by LC–MS/MS and LC–HRMS. •. Immunochromatographic tests provide rapid, cheap, portable, and multiplex analyses. •. Exposure to mycotoxins should be assessed by measuring suitable biomarkers. Mycotoxins are toxic metabolites of certain fungi that growth on a variety of crops, pre ...

Simultaneous detection of 12 mycotoxins in cereals using ...

2011-2-18 · A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, …

Evaluation of QuEChERS for mycotoxin screening

2019-8-21 · QuEChERS extraction with HRMS and accurate mass for the analysis of T-2 and HT-2 in cereals [1]. Even in the absence of known mycotoxins, they might still occur in conjugated form and be undetected by targeted detection techniques. Soluble conjugated mycotoxins have been nominated as "masked" or "modified" mycotoxins.

grinder for cereals mycotoxins determination

Simultaneous determination of 16 mycotoxins in cereals . A method for the simultaneous determination of 16 mycotoxins in major cereal species has been established using an Agilent Triple Quadrupole LC/MS system. Crushed samples of wheat and maize were extracted with an acetonitrile-water-acetic acid solution (70:29:1, v/v/v).

Development and validation of a method based on a …

2010-3-5 · A new analytical method for the rapid and simultaneous determination of five mycotoxins (zearelenone, deoxynivalenol, Fusarenon X, 15-acetyldeoxynivalenol and nivalenol) in breakfast cereals and flours by heart-cutting GC-MS has been developed and validated.

DETECTION METHODS FOR MYCOTOXINS IN CEREAL …

2010-1-27 · the simultaneous determination of T-2 and HT-2 after IAC clean-up in natu-rally contaminated cereal grains (wheat, maize, oats and barley). Several HPLC methods for identifying various mycotoxins in a number of cereals and cereal--based products have been validated by collaborative studies, and their perfor-

Simultaneous Determination of Multi-Mycotoxins in Cereal ...

2017-3-16 · Signal suppression/ enhancement (SSE) effect of 13 mycotoxins in 5 types of matrices. Five levels (1.325, 2.65, 13.25, 26.5, and 53 ng/mL) of standard solutions for each toxin were prepared by ...

Simultaneous determination of major type A and B ...

2015-5-3 · A reliable and sensitive liquid chromatography-tandem mass spectrometric method was developed for the simultaneous quantitative determination in cereals of the Fusarium mycotoxins HT-2 toxin, T-2 toxin, deoxynivalenol, nivalenol and zearalenone, as well as the modified metabolites 3-acetyl-deoxynivalenol, α-zearalenol, β-zearalenol, deoxynivalenol-3 …

Determination of multi-mycotoxins in cereals and of total ...

Determination of multi-mycotoxins in cereals and of total fumonisins in maize products using isotope labeled internal standard and liquid chromatography/tandem mass spectrometry with positive ionization J Chromatogr A. 2017 Mar 24;1490:138-147. doi: 10.1016/j roma.2017.02.027. ...

Current methods of analysis for the determination of ...

2011-2-1 · This article describes the trends in analytical techniques for the determination of trichothecene mycotoxins, namely deoxynivalenol, and T-2 and HT-2 toxins in cereals and cereal products with particular emphasis on screening and rapid approaches. The driving force behind the changing methodologies is mainly attributed to legislative demands.

Determination of mycotoxins

Determination of mycotoxins. As a result of the global marketplace, the safety of food and feed has become of increasing concern for consumers, governments and producers. Several highly publicised worldwide incidents related to chemical …

Simultaneous determination of pesticides, mycotoxins, and ...

A 2D LC-MS/MS method for the simultaneous determination of 350 pesticides, 16 mycotoxins as well as the growth regulators Chlormequat and Mepiquat was developed. The method is applicable to cereals and products thereof. Attention should be paid to the simultaneous analysis of the cereal-relevant myc …

Determination of Regulated and Emerging Mycotoxins in ...

2021-10-5 · Determination of Regulated and Emerging Mycotoxins in Cereals, Nuts, Figs, and Animal Feeds Using Pass-Through SPE and UPLC-MS/MS Nicola Dreolin, Sara Stead, Simon Hird, Timothy Jenkins Waters Corporation Abstract Mycotoxins can occur naturally in a variety of food products via pre- and post-harvest contamination mechanisms and are regulated ...

Determination of Mycotoxins in Cereal-Based Porridge ...

2017-6-28 · An analytical method applying ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in cereal-based porridge destined for infant consumption …

Challenges of Mycotoxins and Their control

2021-5-26 · Basic rapid and easy handling determination methods of mycotoxins commonly use the physicochemical natures of mycotoxins e.g. by fluorescence-stimulating. Presumptive tests like the "black light" test (so called Bright Greenish Yellow Fluorescent (BGYF) - test) shows rapid, indirectly, with less equipment and cost but non-specific mycotoxin ...

Determining Mycotoxins in Baby Foods and Animal Feeds ...

2014-8-25 · We developed a stable isotope dilution assay with liquid chromatography tandem mass spectrometry (LC–MS/MS) to determine multiple mycotoxins in baby foods and animal feeds. Samples were fortified with [13C]-uniformly labeled mycotoxins as internal standards ([13C]-IS) and prepared by solvent extraction (50% acetonitrile in water) and filtration, followed …

Simultaneous determination of 16 mycotoxins in cereals ...

2018-10-12 · A method for the simultaneous determination of 16 mycotoxins in major cereal species has been established using an Agilent Triple Quadrupole LC/MS system. Crushed samples of wheat and maize were extracted with an acetonitrile-water-acetic acid solution (70:29:1, v/v/v). The resulting sample solutions were then separated using

A validated UPLC-MS/MS multi-mycotoxin method for …

2018-6-9 · nuts and cereals ranged 0.08–30.0 and 0.25–99.0 μg/kg, respectively. Validated method was used for monitoring mycotoxins in 120 samples. A total of 97 samples (81%) were contaminated with at least one of these mycotoxins, but only the 3% of the samples exceeded the maximum levels of the EU legislation. ARTICLE HISTORY Received 31 August 2015

Determination of 17 Mycotoxins in Cereals and Cereal …

2021-12-4 · Determination of 17 Mycotoxins in Cereals and Cereal Based Food Using Liquid Chromatography–Triple Quadrupole Mass Spectrometry Download your copy... The goal of the study was to evaluate a dilute-and-shoot approach followed by LC-MS/MS for the analysis of the mycotoxins legislated by the EU as well as some additional mycotoxins of current ...

Simultaneous Determination of Twelve Mycotoxins in …

2015-9-12 · DOI: 10.3724/SP.J.1006.2014.00691 Acta Agronomica Sinica () 2014/40:4 PP.691-701 Simultaneous Determination of Twelve Mycotoxins in Cereals by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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